Research Assistant (Jackson Group) (Fixed Term)
The Steve Jackson laboratory (https://www.stevejacksonlab.org/) at the internationally renowned CRUK Cambridge Institute, https://www.cruk.cam.ac.uk/ (University of Cambridge), is seeking to recruit a bright and enthusiastic Research Assistant.
The postholder will work alongside experienced postdoctoral research associates to assist with several on-going projects in the lab, exploring cellular mechanisms of DNA damage responses and how sensitivity and resistance arise in different cancer cells and genetic backgrounds in response to ionising radiation and clinically relevant drugs.
The postholder will assist with high-throughput CRISPR-Cas9 screens to explore sensitivity/resistance mechanisms in defined genetic backgrounds, followed by validation and ensuing mechanistic studies. Experience in CRISPR genome editing and/or CRISPR genetic screens is essential, as well as a strong background in cell and molecular biology techniques, tissue culture, and stable cell line generation.
The research assistant will be required to take on other responsibilities, including preparation of reagents, maintenance of lab stocks and assisting in the day-to-day running of the laboratory.
The successful candidate should show initiative and independent thinking, possess excellent communication skills and the ability to work independently and as a part of a vibrant and diverse team.
Preferred skills and experience:
¿ Degree or equivalent in life sciences.
¿ Previous experience of working in a molecular cell biology research laboratory required.
¿ Mammalian tissue culture (including transfection, siRNA work and stable cell line generation).
¿ Experience in molecular biology techniques: cloning, PCR, mutagenesis, western blotting, immunofluorescence, DNA and RNA isolation and protein analysis.
¿ Experience in lentivirus production and transduction.
¿ Experience in CRISPR genome editing and/or CRISPR genetic screens.
¿ High attention to detail and good record keeping.
¿ Good communication and organisational skills.
¿ Reliability and enthusiasm.
Click the 'Apply' button below to register an account with our recruitment system (if you have not already) and apply online.
Please ensure that you upload a covering letter and CV in the Upload section of the online application. The covering letter should outline how you match the criteria for the post and why you are applying for this role. Please do not send academic transcripts or certificates.
If you have any questions about this vacancy or the application process, please contact mailbox-hr@cruk.cam.ac.uk.
Fixed-term: The funds for this post are available for 1 years in the first instance.
Once an offer of employment has been accepted, the successful candidate will be required to undergo a basic disclosure (criminal records check) check and a security check.
Click the 'Apply' button below to register an account with our recruitment system (if you have not already) and apply online.
Please ensure that you upload a 2-page Curriculum Vitae (CV), a covering letter and publications list in the Upload section of the online application. If you upload any additional documents that have not been requested, we will not be able to consider these as part of your application.
Please quote reference SW45680 on your application and in any correspondence about this vacancy.
The University actively supports equality, diversity and inclusion and encourages applications from all sections of society.
The University has a responsibility to ensure that all employees are eligible to live and work in the UK.